Table 1.

Simultaneous association of Kifc1 and Kif5B with early endocytic vesicles

Analysis 1
Group 1	No. of ASOR-positive vesicles	301
Group 2	No. in group 1 positive for Kifc1	176
Group 3	No. in group 2 positive for Kif5B	162
	% of Kifc1 vesicles with Kif5B	92%
	% of ASOR vesicles with KifC1 and Kif5B	54%
Analysis 2
Group 4	No. of ASOR-positive vesicles	309
Group 5	No. in group 4 positive for Kif5B	235
Group 6	No. in group 5 positive for Kifc1	161
	% of Kif5B vesicles with Kifc1	69%
	% of ASOR vesicles with KifC1 and Kif5B	52%

Texas Red ASOR-containing early endocytic vesicles from wild-type mouse liver were bound to an optical chamber and immunostained for Kifc1 and Kif5B sequentially as described in Materials and Methods. In the first image analysis (analysis 1), ASOR-containing vesicles were identified (group 1), and those that also contained Kifc1 (group 2) were quantified. Next, the fraction of the group 2 vesicles that also contained Kif5B was quantified. Image analysis of ASOR-containing vesicles was also done in the reverse order (analysis 2).