Figure 1.

Endogenous Scar is present in a large-molecular-weight complex. Clarified cell lysate from growing HPS400 cells was loaded onto a 7–20% sucrose gradient. Fractions of 0.5 ml were collected and immunoblotted for Scar (A) or PIR121 (B). Numbers above blots indicate fraction number with the bottom of the gradient on the left. The majority of endogenous Scar is present in fractions 9–14 (A), which contain protein complexes of ∼400–600 kDa. The majority of PIR121 protein was detected in the same fractions (B).