Figure 5.

Effect of γ2sa on disassembly of hemidesmosome-like structures induced by EGF. Serum-starved HaCaT cells were inoculated in serum-free medium and incubated for 8 h on culture dishes that had been coated with 1.0 μg/ml Pr-LN5 or Np-LN5. The cultures were then treated for 10 min with 0.4 μg/ml soluble γ2sa (γ2sa) in the presence (+EGF) or absence (None) of 0.05 μg/ml EGF. The treated cells were subjected to immnofluorescence staining with an anti-BP180 mAb (A) and the anti-integrin-β4 mAb (B). Other experimental conditions are described in Materials and Methods.