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. 2007 May;18(5):1723–1733. doi: 10.1091/mbc.E06-09-0815

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© 2007 by The American Society for Cell Biology

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Figure 1. — Expression of muscle-specific markers during differentiation of C2C12 cells. Phase contrast images of C2C12 cells grown in proliferation medium (A) and placed in differentiation-promoting conditions for 8 d (B). Different muscle markers were detected by Western immunoblotting in proliferating (d0) as well as in differentiating C2C12 cells, 2 d (d2), 5 d (d5), and 8 d (d8) after inducing differentiation: (C) myogenin; (D) α-actinin 2; (E) myosin; and (F) the 2 ALP isoforms, skALP and smALP.