Figure 7.
The C-terminal region of Oxa1 is not required for the Atp9-Oxa1 interaction. (A and B) Mitochondria were isolated from yeast strains expressing Oxa1, Oxa1His, or Oxa1Δ83His derivatives under control of the GAL10 promoter. (A) In organello translation in the presence of [35S]methionine was performed in the mitochondria, and samples were further analyzed as described in Figure 6. (B) Immunoblotting of Atp5 was performed, in addition to Atp6 and Atp9, and the presence of Oxa1His and Oxa1Δ83His on the Ni-NTA beads were detected using an Oxa1 antibody specific for the N-terminal region of Oxa1.