Table 1.

Sequence of primers and probes used for Pfcrt and Pfmdr1 amplification and melting temperatures of the sensor probes of each allele

	Melting temperatures (°C) of the sensor probes
Sequence 5' to 3'	Wild	Mutant
Pfcrt
Forward Primer: CTTGTCTTGGTAAATGTGCTCA
iLC Primer: GTTACCAATTTTGTTTAAAGTTCT
Sensor Probe: TGTGTAATTGAAACAATTTTTGCTAA	46.5 ± 0.2	65.3 ± 0.4
Pfmdr1
Forward Primer: TGTATTATCAGGAGGAACATTACC
Reverse Primer: ACCACCAAACATAAATTAACGGA
Sensor Probe 86: ATTAATATCATCATAAATACATG	51.8 ± 0.3	56.5 ± 0.2
Anchor Probe 86: TCTTTAATATTACACCAAACACAGATAT
Sensor Probe 184: TAAAAAATGCACGTTTGACTTTATGTATTA	53.0 ± 0.2	58.7 ± 0.3
Anchor Probe 184: CCTTTTTAGGTTTATTTATTTGGTCAT