Table 1.
Primer pair sequences, accession numbers, amplicon size and annealing temperature conditions for genes of interest used for real-time PCR.
| Target Gene | Primer sequence* | Amplicon size (nucleotides) | Annealing temperature (°C) | GenBank accession number | |
| Forward | Reverse | ||||
| ERα | TCCAGGAGCTGTCTCTCCAT | GATCTCAGCCATACCCTCCA | 173 | 55 | DQ009007 |
| ERβ | GAGCATCCAAGGTCACAATG | CACTTTGTCATGCCCACTTC | 126 | 59 | AY508959 |
| Vtg | AAGCCACCTCCAATGTCATC | GGGAGTCTGTCCCAAGACAA | 391 | 57 | DY802177 |
| Zr-protein | TGACGAAGGTCCTCAGGG | AGGGTTTGGGGTTGTGGT | 113 | 55 | AF407574 |
| Vigilin | GGGATACGCACAGACACCTT | CCCAGATTCCACAGACACCT | 86 | 60 | DY802195 |
| AhRα | AGGGGCGTCTGAAGTTCC | GTGAACAGGCCCAACCTG | 82 | 60 | AY219864 |
| AhRβ | GACCCCCAGGACCAGAGT | GTTGTCCTGGATGACGGC | 96 | 65 | AY219865 |
| AhRR | TTCCTCCAGGGACAGAAGAA | ATGGAGGGCAGCAGAAGAG | 98 | 60 | DQ372978 |
| Arnt | AGAGCAATCCCAGGGTCC | TGGGAGGGTGATTGAGGA | 107 | 60 | DQ367887 |
| CYP1A1 | GAGTTTGGGCAGGTGGTG | TGGTGCGGTTTGGTAGGT | 76 | 60 | AF364076 |
| UDPGT | ATAAGGACCGTCCCATCGAG | ATCCAGTTGAGGTCGTGAGC | 113 | 55 | DY802180 |
| Proteasome | TCTTTGACCAGGTTGCACAG | CATACAAAGCTGGTGGCTCA | 134 | 60 | DY802110 |