Table 1.

Cortical Cataracts in Transgenic Mice Compared with Age-Matched Nontransgenic Control Mice

			Grade of Opacity
Transgenic Line	Genotype	Lenses (n)	Grade 0	Grade I	Grade II	Grade III
Line 8168 (R116C)	Tg*	54	20 (37)	21 (39)	11 (20)	2 (4)
	Control	50	37 (74)	9 (18)	4 (8)	0 (0)
Line 8165 (R116C)	Tg*	74	29 (39)	24 (32)	14 (19)	7 (10)
	Control	46	32 (69)	10 (22)	4 (9)	0 (0)
Line 8170 (R116C)	Tg*	72	17 (23)	26 (36)	20 (28)	9 (13)
	Control	62	34 (55)	18 (29)	10 (16)	0 (0)
Line 10694 (wild type)	Tg	62	18 (29)	38 (61)	4 (7)	2 (3)
	Control	70	21 (30)	42 (60)	7 (10)	0 (0)

Lenses were examined ex vivo by stereomicroscopy under dark-field illumination. Cataracts were graded according to the percentage of posterior lens surface covered by opacity. Grades were 0, no opacity; I, < 20% coverage; II, 20%–50% coverage; III, > 50% coverage. Tg, transgenic animals; controls were age-matched nontransgenic littermates. Opacity scores were not significantly different among lenses examined at the ages of 9 to 11 weeks, 20 to 22 weeks, and 28 to 30 weeks. Therefore, data from all age groups were pooled. Data are number of lenses, with percentage of total group in parentheses. For the purpose of statistical analyses, grades II and III are combined. Test results for interaction between level of gene expression and severity of cortical cataract (transgenic group only) were not significant (P = 0.22).

^*Distribution of severity of lens defect differed between transgenics and control mice (P < 0.05).