Table 2.

Abundance of Swollen Cortical Fibers in Transgenic Mice Compared with Age-Matched Nontransgenic Control Mice

			Grade of Opacity
Transgenic Line	Genotype	Lenses (n)	Grade 0	Grade I	Grade II	Grade III
Line 8168 (R116C)	Tg	54	11 (20)	18 (33)	17 (32)	8 (15)
	Control	50	16 (32)	19 (38)	13 (26)	2 (4)
Line 8165 (R116C)	Tg*	74	7 (9)	23 (31)	31 (42)	13 (18)
	Control	46	20 (44)	16 (35)	8 (17)	2 (4)
Line 8170 (R116C)	Tg*	72	4 (5)	13 (18)	38 (53)	17 (24)
	Control	62	6 (10)	28 (45)	28 (45)	0 (0)
Line 10694 (wild type)	Tg	62	7 (11)	30 (48)	22 (36)	3 (5)
	Control	70	14 (20)	31 (44)	22 (32)	3 (4)

Lenses were examined ex vivo by stereomicroscopy under bright-field illumination. Swollen fiber phenotypes were graded according to the percentage of posterior lens surface covered by swollen fibers. Grades were 0, no swollen fibers; I, < 20% coverage; II, 20%–50% coverage; III, > 50% coverage. Tg, transgenic animals; controls were age-matched nontransgenic littermates. Scores were not significantly different among lenses examined at the ages of 9 to 11 weeks, 20 to 22 weeks, and 28 to 30 weeks. Therefore, data from all age groups were pooled. Data are number of lenses, with percentage of total group in parentheses. For the purpose of statistical analyses, grades II and III are combined. Test results for interaction between level of gene expression and severity of swollen cortical fibers (transgenic group only) were significant (P = 0.03).

^*Distribution of severity of lens defect differed between transgenic and control mice (P < 0.05).