TABLE 1.
Mad2-dependent mitotic arrest assessed in the cut7 mutant
| mad2 allele in host cell | mad2 allele on plasmid | % of cells with condensed chromosomes (total no. of cells) |
|---|---|---|
| mad2Δ | mad2+ | 44.0 (184) |
| None | 2.2 (180) | |
| mad2-56 | 4.6 (151) | |
| mad2-64 | 6.7 (180) | |
| mad2-64M52I | 42.9 (163) | |
| mad2-64D177V | 31.6 (190) | |
| mad2+ | mad2+ | 67.2 (119) |
| None | 35.6 (174) | |
| mad2-56 | 7.8 (255) | |
| mad2-64 | 7.8 (218) | |
| mad2+ | No plasmid | 52.7 (163) |
| mad2Δ | 1.7 (241) | |
| mad2+∷kanr | 53.2 (171) | |
| mad2-56∷kanr | 5.2 (271) | |
| mad2-64∷kanr | 6.5 (245) |
Strains containing plasmid were incubated at 36° for 4 hr in minimal medium, but those without plasmid were incubated in YPD medium at 36° for 3 hr. Cells were fixed with glutaraldehyde and stained with DAPI. Cells that had hypercondensed chromosomes were scored. Yeast strains used were P43 (mad2Δ) and HM783 (mad2+) for the tests with plasmids, and P43, HM783, YT541 (mad2+∷kanr), YT542 (mad2-56∷kanr), and YT543 (mad2-64∷kanr) for chromosomal mad2 alleles.