TABLE 2.

Effect of mad2 mutations on minichromosome stability

mad2 allele at chromosomal locus	mad2 allele on plasmid	TBZ	Total no. of colonies	% half-sectored colonies
mad2Δa	mad2+	0 μg/ml	247,165	0.032
	None		250,158	0.19
	mad2-56		260,861	0.047
	mad2-64		266,571	0.029
	mad2+	7.5 μg/ml	41,076	0.054
	None		7,066	1.8
	mad2-56		40,922	0.43
	mad2-64		36,916	0.21
mad2+b	mad2+	0 μg/ml	38,422	0.005
	None		36,545	0.012
	mad2-56		44,037	0.014
	mad2-64		36,188	0.006
mad2+∷kanrc	NAd	0 μg/ml	72,048	0.011
mad2Δ			53,651	0.15
mad2-56∷kanr			33,853	0.035
mad2-64∷kanr			64,886	0.020
mad2+∷kanr		7.5 μg/ml	66,316	0.030
mad2Δ			6,170	0.91
mad2-56∷kanr			16,994	0.78
mad2-64∷kanr			31,399	0.26

^ah⁻ leu1 ade6-210 mad2Δ Ch16 (P112; see supplemental Table 1 at http://www.genetics.org/supplemental/) was transformed with a plasmid carrying the indicated mad2 alleles. Minichromosome stability assay was performed on synthetic medium selective for the plasmid marker.

^bSame as in footnote a, but P113 was used (h⁻ leu1 ade6-210 Ch16).

^ch⁻ leu1 ade6-210 Ch16 with the indicated mad2 alleles at the chromosomal loci were used to determine minichromosome stability on YE plates. Strains used were P224, P112, P225, and P227.

^dNA, not applicable.