Figure 5.—

Nup154 and Cup functionally interact during egg-chamber development. Egg chambers from single (A, D, G and B, E, H) or double (C, F, and I) mutants were stained with DAPI and analyzed by conventional epifluorescence. (A) Ovariole from a tlp² homozygote. (B) Ovariole from a cup¹³⁵⁵ homozygote. Egg-chamber maturation proceeds normally in both mutants. (C) Ovariole from a cup¹³⁵⁵, tlp² double homozygote, showing a “string of pearls” morphology due to the arrest of egg-chamber development around stage 4–5. (D) Stage 7 egg chamber from a tlp² homozygote. Slight alterations of the nurse-cell chromatin structure are present only in the two most posterior nurse cells, which also display undersized nuclei (one of them is clearly visible in this focal plane). (E) Stage 7 egg chamber from a cup¹³⁵⁵ homozygote. Nurse-cell chromosomes undergo normal dispersal in this mutant allele. (F) Stage 7 egg chamber from a cup¹³⁵⁵, tlp² double homozygote. Virtually all nurse-cell nuclei display aberrant chromatin configurations, mainly represented by “blob like” and “spiral” chromosomes. (G) Ovariole from a tlp¹ homozygote. (H) Ovariole from a cup²¹ homozygote. (I) Ovariole from a cup²¹, tlp¹ double homozygote.