Figure 7.—
Overexpression of SSH4, RCR2, and RCR1 affects multiple plasma membrane transport systems. (A) Suppression of pho86Δ. Serial dilutions of PHO86 (CEN.PK113-5D/PHO84myc) and pho86Δ strains (JKY6) carrying pRS202 (vector) or plasmids expressing SSH4 (pJK123), RCR2 (pJK124), or RCR1 (pJK125) from the PADH1 promoter, as indicated, were spotted onto high-phosphate (YPD) and low-phosphate (SD 0.2 mm phosphate) media. Plates were incubated at 30° for 3 days and photographed. (B) Growth characteristics on FOA-containing media. Wild-type strain CAY28 was transformed with 2μ plasmids pRS202 (vector), pJK120 (SSH4), pJK123 (ADH1-SSH4), pJK121 (RCR2), pJK124 (ADH1-RCR2), pJK122 (RCR1), or pJK125 (ADH1-RCR1) and 10-fold dilution series were spotted onto SD and SD+FOA (0.1 mg/ml). Plates were incubated at 30° for 3 days and photographed.