TABLE 5.
mcd-1 loss of function causes synthetic lethality with some class B synMuv mutations
| Genotype | Growth phenotypea |
|---|---|
| A. Some class B and C synMuv mutations cause synthetic growth and viability defects with mcd-1(n4005) | |
| mcd-1(n4005) | Viableb |
| lin-53(n833); mcd-1(n4005)c | Slow growthd |
| mcd-1(n4005); lin-13(n770) | Slow growthd |
| mcd-1(n4005); lin-54(n2231) | Slow growthd |
| dpl-1(n3380) mcd-1(n4005)e | Slow growthdf |
| mcd-1(n4005); lin-9(n112) | L1 arrestg |
| lin-35(n745); mcd-1(n4005) | L1 arrestg |
| mcd-1(n4005); lin-37(n758) | L1 arresth |
| mcd-1(n4005); mys-1(n3681) | Slow growthd |
| B. Some class B synMuv mutations do not cause synthetic lethality with mcd-1(n4005) | |
| mcd-1(n4005); lin-36(n766) | Viable |
| mcd-1(n4005); lin-52(n771) | Viable |
| mcd-1(n4005); tam-1(cc567)i | Viable |
| C. Class A synMuv mutations do not cause synthetic lethality with mcd-1(n4005) | |
| lin-8(n2731) mcd-1(n4005) | Viable |
| mcd-1(n4005); lin-15A(n767) | Viable |
| lin-38(n751) mcd-1(n4005) | Viable |
| lin-56(n2728) mcd-1(n4005) | Viable |
The growth phenotype was assayed at 20° and 25° by picking L4 larvae and quantifying the time until >50% of the next generation reached the L4 larval stage.
Viable strains can be maintained and have a growth phenotype of between 3 and 3.5 days.
Strain also contained dpy-5(e61).
The development of this strain was delayed 2 days as compared to the mcd-1 single-mutant strain or the respective class B synMuv mutant strain at 20°.
Strain also contains unc-4(e120).
dpl-1(n3380) unc-4 mcd-1(n4005) animals were homozygous viable and slow growing at 20°. At 25° the strain could not be maintained after 24 days with infertile adults, sick slow-growing larvae, and some arrested larvae.
One hundred percent of the animals arrested as larvae in size similar to that of L1 larvae, whereas the mcd-1 single-mutant and the respective class B synMuv mutant strains do not.
At 20° animals were small, infertile Muv adults, sick slow-growing larvae, or arrested larvae. At 22.5° and 25°, 100% of the animals arrested as larvae in size similar to that of L1 larvae.
Strain also contained unc-46(e177).