Figure 3.—

The timing of mitosis in two-cell stage embryos. (A–C) Images of the wild-type, unc-46(e177) mdf-1(gk2) h1985, and unc-46(e177) mdf-1(gk2); fzy-1(h1983) two-cell embryos from Figure 2. The T = 0 min time point shows cells during NEBD in the AB cell. The embryos were imaged every 2 min from this point until the formation of the four-cell embryo (bar, 20 μm). (D) Summary of the mitotic timing data from live-cell imaging. Bars represent the duration of mitotic division from NEBD to NER in two-cell embryos. Mean durations are plotted in seconds with SEM error bars (n = 10 measurements for each strain). The data for h1987, h1958, and h1985 as well as the previously known suppressors emb-30(tn377ts) and fzy-1(h1983) are included. All the measurements were performed at 20°, except for emb-30(tn377ts) and wild-type control strains observed at 16°.