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. 2007 Apr;175(4):1615–1624. doi: 10.1534/genetics.106.068106

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Copyright © 2007 by the Genetics Society of America

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Figure 4.— — flam mutations affect germline cell development. Ovaries were stained with DAPI. (A) A 24-hr-old wild-type ovariole contains a long string of developing egg chambers produced continuously by the germarium (Ge) and connected to each other by stalk cells (Sc). (B) flam^BG(P) and (C) flam^KG(P) are typical 24-hr-old ovaries. They are very small and contain a number of rudimentary ovarioles within which no differentiation of germ cells has taken place (arrows). In C two egg chambers exhibit gaps in the FE (arrowheads). (D) flam^BG(P) 48-hr-old ovarioles are severely dystrophic. Ovarioles contain two to three completely disorganized egg chambers. These chambers contain a reduced number of abnormally shaped nuclei. (E) Enlargement of the portion of ovariole shown in D, delimited by brackets. The star indicates an egg chamber containing a single nurse cell-like nucleus. (F) A wild-type mature egg. (G) A mutant flam^BG(P) mature egg lacks polarity and has abnormal dorsal appendages (da). Bars, 80 μm.