Figure 5.—

Soma-dependent FE defects of flam mutants. (A) In 6-day-old flam^BG(P) ovaries, follicle cells form aggregates (arrowheads) while large gaps are seen elsewhere in the FE (arrow). (B) Forty-eight-hour-old flam^KG(P) ovarioles. Follicle cells fail to invaginate in the germarium, resulting in a compound egg chamber with nurse cells that present various degrees of polyploidization (arrows). (C) A 3-day-old flam^py+(P) ovary. Abnormalities are frequently observed during ovarian development. Here a compound egg chamber is present (arrow). Also present is an egg chamber with a deficit in nurse cell number (arrowhead). (D) Stage 11 egg chamber from a 3-day-old flam^py+(P) female in which follicle cells have not migrated properly, resulting in nurse cells being pinched off into the ooplasm. Follicle cells accumulate at the anterior of the egg (arrow). (E) A pair of ovaries containing ovarioles with homozygous flam^BG(P) germline clones and ovarioles from ovo^D1 flam⁺ germline cells. The ovo^D1 flam⁺ germline cells are arrested at the beginning of oogenesis because of the ovo^D1 mutation (arrows). By contrast, in ovarioles in the left ovary, containing flam^BG(P) germline clones, germline stem cells divided normally. Bars, 80 μm.