Figure 5.—

Multi-copy MID2 and PKC1-containing plasmids improve the temperature-sensitive growth defects of select chaperone mutants. ssa1-45 or hsp82 G313N yeast strains were transformed with multi-copy MID2-HA- or PKC1-containing vectors or with an empty vector. These strains and isogenic strains containing a wild-type version of the mutated genes were serially diluted 10-fold on SC–ura for 3–4 days at the indicated temperatures. (A) MID2-HA and PKC1 do not rescue the temperature-sensitive growth phenotype of ssa1-45 yeast. (B) Overexpression of Mid2p-HA (left) and Pkc1p (right) in the ssa1-45 strain were verified by immunoblot analysis. (C) High-copy MID2-HA and PKC1 improve the growth defect of the hsp82 G313N strain.