Figure 6.—

The ydj1Δ and hsp82 temperature-sensitive mutant strains have phenotypes consistent with defects in cell-wall synthesis. (A) (Top) Tenfold serial dilutions of ydj1Δ yeast either expressing Ydj1p (“YDJ1”) or lacking the expression vector were plated onto YPD either lacking or containing 0.4 m NaCl, 1 m sorbitol, or 20 μg/ml calcofluor white (CW) at the indicated temperatures for 3 days. (Middle and bottom) Wild-type or ydj1Δ yeast were incubated on control or sorbitol or CW-containing medium as above. (B) HSP82 and hsp82 yeast were grown on either YPD or YPD with either 1 m sorbitol or 20 μg/ml CW at the indicated temperatures for 3 days. All the yeast strains are ade⁻ and have a pink coloration. ydj1Δ yeast also obtained the pink coloration after longer incubations (not shown).