P-values (one-tailed test) of the Λ statistic under different bottleneck scenarios (A) C = 11.7, f = 1. (B) C = 50, f = 1. Contours represent the confidence region of parameter space with P-values from P < 0.01 (darker shading) and increasing in units of 0.01 (to the lighter shading). Times are scaled in units of 4Ne generations, 10 generations/year. The standard constant-size neutral model is rejected by the Λ statistical test (C = 11.7, P = 0.002; C = 50, P < 10−4).