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. Author manuscript; available in PMC: 2008 Jan 19.
Published in final edited form as: J Mol Biol. 2006 Oct 3;365(3):680–693. doi: 10.1016/j.jmb.2006.09.079

Figure 4.

Figure 4

Mutagenesis study of Ala57 in Bit IgV. Residue Ala57 in Bit IgV was changed to various amino acids as described in the Methods. After DNA sequencing confirmed the amino acid changes, the mutant Bit.IgV-Fc fusion proteins were expressed in COS cells, purified and tested for binding to CD47-AP.