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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Anal Biochem. 2006 Mar 31;353(2):209–216. doi: 10.1016/j.ab.2006.03.020

Figure 2.

Figure 2

Restriction enzyme mediated release of PRPs. Panel A shows dark field images of PRPs bound to biotinylated DNA fragment attached to a solid surface. Addition of EcoR1 results in the release of PRPs from the surface (T20 = 20 minutes, +EcoR1). In the absence of specific enzyme cleavage of the linker DNA, PRPs are retained on the surface (T20, −EcoR1). The time course of PRP release following addition of restriction enzyme is shown in Panel B. The error bars calculated for each time point are based on the detection and counting of the number of PRPs released from three separate designated imaging areas.