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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Anal Biochem. 2006 Mar 31;353(2):209–216. doi: 10.1016/j.ab.2006.03.020

Figure 4.

Figure 4

Detection of an enzyme inhibitor using PRP release assay. The immobilized linker DNA is incubated with 1 U of EcoR1 methyltransferase per reaction in the presence of increasing concentrations of sinefungin, a specific methyltransferase inhibitor. The inhibition of methyltransferase is detected by the larger number of PRPs that are released from samples incubated with higher concentrations of the inhibitor. The inset shows an ethidium bromide stained gel of fragments generated after solution phase incubation with 1 U EcoR1 methyltransferase, in the presence or absence of 100 μM sinefungin, followed by EcoR1 digestion.