Table 1.

Library screening overview

^aWhen alternative splice forms were annotated, the longest open reading frame is shown.

^bSome amplicons cover multiple exons.

^cThe number of 384-well plates that were actually screened for a gene. Although the library consists of 16 384-well plates, we did not screen the complete library for all amplicons.

^dOnly bases with a Phred score of ≥20 were included in the analysis.

^ePercent of library screened is calculated by dividing the total number of exonic bases read by the multiplication of the open reading frame length of a gene with the total number of mutants (6144) in the library.

^fThe number of non-G/C to A/T mutations is shown in parentheses.

^gMutations that could not be reconfirmed in an independent PCR and resequencing assay. The number of non-G/C to A/T mutations is shown in parentheses.