Table 3.

Genome size and amount of nonsense introducing mutations (NIMs) for various organisms

^aData are based on Ensembl version 41: C. elegans, WS160; mouse, NCBIM36; rat, RGSC3.4; zebrafish, Zv6; Arabidopsis, TAIR6.0; human, NCBI36.

^bIn the case of alternative splicing, the longest coding ORFeome was used.

^cThe percentage of the mutation frequency spectrum used for the calculations is shown in parentheses.

^dAll genomic positions that can be changed into a stop codon (NIMs), given the mutagen/spectrum defined in column 2.

^eAll positions in the consensus splice site sequences (ag. . .gt) that can be mutated, given the mutagen/spectrum defined in column 2.

^fSum of all the NIMs and splice site positions that can be mutated.

^gMost NIMs are monoallelic, but in some cases two different mutations at the same genomic position (e.g., TAC to TAG and TAA) contribute to the genome-wide collection.

^hNumber of genomic positions divided by the number of NIMs. The number in parentheses is the number when splice sites are taken into account as well.

ⁱOnly G to A mutations.

^jFull spectrum: G/C to A/T, A/T to G/C, A/T to T/A, and A/T to C/G (De Stasio and Dorman 2001).

^kPartial spectrum: G/C to A/T, A/T to G/C, and A/T to C/G.

^lEvery possible mutation is considered to be possible.

^mPartial spectrum: A/T to T/A, A/T to G/C, and G/C to A/T (Noveroske et al. 2000).

ⁿPartial spectrum: A/T to T/A, A/T to G/C.

^oPartial spectrum: A/T to T/A, A/T to G/C, G/C to A/T, and G/C to T/A (Smits et al. 2006).

^pPartial spectrum: A/T to T/A, A/T to G/C, G/C to A/T, and G/C to T/A (Wienholds et al. 2003).

^qPartial spectrum: A/T to T/A, A/T to G/A, and G/C to A/T.

^rPartial spectrum: G/C to A/T (Greene et al. 2003).