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. 2007 May;17(5):586–593. doi: 10.1101/gr.6085507

Figure 3.

Figure 3.

(A) Comparison of amino acid sequences of Nr6a1 hinge domains of pig, human, and mouse. We identified an amino acid substitution (Pro192Leu; C → T at nucleotide 748 of AB248749) in pig NR6A1. Leucine occurred in alleles increasing vertebral number (Ver), whereas proline occurred in the wild-type pig allele (wt) as well as in human and mouse NR6A1/Nr6a1. (B) Effect of an amino acid substitution in pig Nr6a1 on its interaction with RAP80 and NCOR1. Interaction of pig NR6A1 and its corepressors RAP80 and NCOR1 was analyzed with a two-hybrid system in mammalian cells. CHO cells were transfected with pACT and pBIND plasmids containing the indicated DNA fragments. After 48 h, cells were collected and assayed for reporter activity. Relative luciferase activity was plotted and compared with the results of t-tests; error bars, 1 SD.