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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: J Cereb Blood Flow Metab. 2006 Mar 1;26(10):1323–1331. doi: 10.1038/sj.jcbfm.9600286

Figure 4.

Figure 4

Effect of preconditioning on P-murine double minute 2 (MDM2) in PC12 cells exposed to oxygen and glucose deprivation (OGD). (A) Representative Western blot of P-MDM2 levels in cells exposed to OGD for 0, 2, 4, and 6 h without and with preconditioning (lanes 1, 2, 3, and 4, and lanes 5, 6, 7, and 8, respectively). Reblotting with anti-β-actin was performed as loading control. (B) Quantitative densitometric analysis of the effect of preconditioning on P-MDM2 levels in PC12 cells exposed to OGD. Results represent means + s.e.m. (n = 4) of P-MDM2 levels in cells exposed to OGD for 0, 2, 4, and 6 h without and with preconditioning (OGD and PC + OGD, respectively). A significant difference was obtained among the values of preconditioned and non-preconditioned cells during 0 and 2 h of OGD. *P < 0.05 and ***P < 0.001, compared with OGD values; bifactorial repeated measures analysis of variance.