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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: J Cereb Blood Flow Metab. 2006 Mar 1;26(10):1323–1331. doi: 10.1038/sj.jcbfm.9600286

Figure 6.

Figure 6

Effect of the phosphoinositide 3-kinase (PI3K) inhibitor LY294002 and preconditioning on apoptosis oxygen and glucose deprivation (OGD)-induced apoptosis in PC12 cells. Results represent means + s.e.m. (n = 5) of the percentage of total of viable, early apoptotic (E apop), medium–late apoptotic (M–L apop) and necrotic (necro.) cells quantified by fluorescence-activated cell sorter (FACS) analysis using propidium iodide (PI) and Hoechst 33342 labeling. Preconditioning significantly increased the percentage of viable cells and decreased the percentage of apoptotic cells compared with OGD alone. LY294002 (10 μmol/L) significantly increased the number of viable cells compared with OGD and significantly potentiated the protective effect of PC. *P < 0.05 and ***P < 0.001, compared with OGD values; +P < 0.05 and + +P < 0.001, compared with PC + OGD; repeated measures analysis of variance.