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. Author manuscript; available in PMC: 2007 Apr 24.

Published in final edited form as: Arch Biochem Biophys. 2006 Mar 23;453(1):63–69. doi: 10.1016/j.abb.2006.03.004

Fluorescence emission spectra of the single-Trp variants of ClpB. The excitation wavelength was 300 nm and the emission spectra were measured for each of the variants of ClpB indicated in the figure at 0.1 mg/ml in buffer A (50 mM Tris/HCl pH 7.5, 20 mM MgCl₂, 1 mM EDTA, 1 mM DTT) with 0.3 M KCl (A) or at 0.5 mg/ml in buffer A without KCl (B).

Fluorescence emission spectra of the single-Trp variants of ClpB. The excitation wavelength was 300 nm and the emission spectra were measured for each of the variants of ClpB indicated in the figure at 0.1 mg/ml in buffer A (50 mM Tris/HCl pH 7.5, 20 mM MgCl₂, 1 mM EDTA, 1 mM DTT) with 0.3 M KCl (A) or at 0.5 mg/ml in buffer A without KCl (B).