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. Author manuscript; available in PMC: 2008 Feb 15.
Published in final edited form as: Gene. 2006 Oct 17;388(1-2):74–82. doi: 10.1016/j.gene.2006.10.003

Figure 5.

Figure 5

Electrophoretic Mobility Shift Assays (EMSAs) showing the site 1 and site 2 specifically bind Sp1. EMSAs were performed using 24 bp oligonucleotides identical to site 1 and site 2 with HeLa nuclear extracts. (A.) Lanes 1 and 6 are HeLa nuclear extract alone. Lanes 2 and 7 show specific competition for binding with an Sp1 consensus oligonucleotide. Lanes 3 and 8 show competition with unlabeled site 1 and site 2 oligonucleotides (s1 and s2 respectively), respectively. Lanes 4 and 9 show competition with site 1 and site 2 mutant oligonucleotides (ms1 and ms2 respectively). Lanes 5 and 10 show non-specific competition with an TFIID consensus oligonucleotide. The unlabelled competitor oligonucleotide added to the binding reactions are indicated on top on each lane. (B.) An Sp1 antibody causes a specific supershift of the bound proteins in site 1 and site 2. Lane 1 is free probe. Lanes 2 and 3 were probed with the Sp1 consensus oligonucleotide. Lanes 4 and 5 were probed with the site 1 oligo. Lanes 6 and 7 were probed with the site 2 oligonucleotide.