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Methods used for RNA interference (RNAi) in C. elegans. A plasmid with opposing T7 promoter sequences containing “your favorite gene (YFG)” is constructed. The plasmid can be used in two ways. The top of the figure shows dsRNA made by in vitro transcription that is used for either soaking or injecting the animals. The bottom of the figure shows how animals can be fed bacteria that are producing dsRNA in vivo.
