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. Author manuscript; available in PMC: 2008 Jan 1.

Published in final edited form as: J Mol Cell Cardiol. 2006 Nov 1;42(1):159–176. doi: 10.1016/j.yjmcc.2006.09.012

H₂O₂ pretreatment reduces Dox-induced apoptosis. Serum-starved CMCs were treated with 100 μM H₂O₂ for 1 hr. The cells recovered for 24 hr in fresh DMEM containing 0.5 % FBS before treatment with different doses of Dox (A) or 0.6 μM Dox (B & C). Caspase-3 activity (A), cell detachment (B) and Annexin V binding (C) were measured 16 hr after addition of Dox. The relative fluorescent unit (RFU) was corrected for protein content to indicate caspase-3 activity (A). At least 300 cells were scored from each view under a phase contrast microscope and three views were chosen randomly for scoring the proportion of detached versus attached cells (B). A letter indicates a significant difference (p<0.05) from the means labeled with a different letter as determined by ANOVA followed by Bonferroni analysis (A, B).

H₂O₂ pretreatment reduces Dox-induced apoptosis. Serum-starved CMCs were treated with 100 μM H₂O₂ for 1 hr. The cells recovered for 24 hr in fresh DMEM containing 0.5 % FBS before treatment with different doses of Dox (A) or 0.6 μM Dox (B & C). Caspase-3 activity (A), cell detachment (B) and Annexin V binding (C) were measured 16 hr after addition of Dox. The relative fluorescent unit (RFU) was corrected for protein content to indicate caspase-3 activity (A). At least 300 cells were scored from each view under a phase contrast microscope and three views were chosen randomly for scoring the proportion of detached versus attached cells (B). A letter indicates a significant difference (p<0.05) from the means labeled with a different letter as determined by ANOVA followed by Bonferroni analysis (A, B).

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