Figure 2.

Representative live H2B–GFP images of different meiotic stages from wild-type, air-2(RNAi), and Ceglc-7 α/β(RNAi) embryos. (A–E) Schematic drawing of meiotic chromosomes in the oocyte-derived nucleus in a wild-type embryo. Each bivalent is represented as two short rods joined end to end with the outer ends leading toward poles. Homologues from one bivalent are indicated by different shades of gray in A–C. After the extrusion of a polar body (pb), sister chromatids are shown as small short rods in D and E. (F–J) Wild-type; (K–O) air-2(RNAi); (P–T) Ceglc-7α/β(RNAi) meiotic embryos. (A, F, and K) Initial alignment, parallel to the cell cortex. (B and G) After a 90° rotation, the chromosomes are perpendicular to the edge of the embryo. (C and H) Anaphase I; (D and I) metaphase II; (E and J) anaphase II. Embryos in K and P, L and Q, M and R, N and S, and O are at the same stage as those in F, G, H, I, and J, respectively. All images presented here are representative slices from each time point except for that in T, which is a projection of the image stack of DAPI staining from a fixed embryo. The edge of the embryo is indicated by a black curve in A–E and a white curve in F–T. Bar, 2.5 μm.