Figure 4.
Subchromosomal localization of AIR-2 and BIR-1 is dependent on chiasma formation. (A and B) Schematic drawing of the bivalent in vertebrates (A) and C. elegans (B). Sister chromatids are shown in the same color, whereas homologues are represented in different colors. The direction of pulling force is indicated by arrows. Green and orange bars represent cohesin molecules connecting chromosome arms distal and proximal, respectively, to chiasmata. (C–F) Merged and projected images of chromosomes from the −1 oocyte of him-5(e1490) (C and E) and her-1(e1518) XO (D and F) animals stained with either AIR-2 (C and D) or BIR-1 (E and F) antibodies. Arrows point to univalents, and arrowheads point to representative bivalents. Bar, 2 μm.