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. 2002 Apr 15;157(2):219–229. doi: 10.1083/jcb.200110045

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Ectopic localization of AIR-2 in Ceglc-7 α/β(RNAi) animals. Representative proximal gonads from wild-type (A) and Ceglc-7α/β(RNAi) (B) animals stained with AIR-2 (green) and DAPI (red) and shown as a merged image. Numbers above A indicate positions of oocytes with −1 being the most proximal. In >95% of wild-type gonads examined, chromosomal AIR-2 is detected only in the −1 oocytes. In 100% of Ceglc-7α/β(RNAi) animals, chromosomal AIR-2 is detected in 2–5 proximal oocytes. (C–F) High magnification of selected bivalents from wild-type (C and D) and Ceglc-7α/β(RNAi) (E and F) oocytes stained with AIR-2 (C and E) or DAPI (D and F). Arrows point to the poleward axes, and arrowheads point to the equatorial axes. (I) A schematic model for how AIR-2 regulates the release of cohesion in meiosis I. Orange bar, unphosphorylated REC-8; red triangle, AIR-2; large green bar, phosphorylated REC-8; small green bars, degraded REC-8. Bars: (B) 5 μm; (F) 1 μm.