Figure 1.

Induction of glycolysis by hypoxia is dependent on growth factor signals. (A) Cells were cultured in the presence or absence (3 wk) of IL-3. Following 24 h incubation at 21% O₂ or 1.5% O₂, cellular glucose utilization was measured by the conversion of 5-³H-glucose to ³H₂O. Data represent mean of three independent experiments ± SD. (B) Glucose-dependent lipid synthesis was measured by labeling cells with D-[U-¹⁴C₆] glucose for 24 h. Lipid extracts were obtained from 2 × 10⁶ cells as described in the Materials and Methods. Data are a representative experiment performed in triplicate ± SD.