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. 2007 May 1;21(9):1037–1049. doi: 10.1101/gad.1529107

Figure 5.

Figure 5.

Cellular proliferation is controlled by the levels of HIF-1α. (A) Growth curves of primary T cells isolated from Hif-1αfl/fl Cre− (wild-type [WT]) or Hif-1fl/fl Cre+ (Cre) mice that were administered tamoxifen. Two independent experiments were performed and the data presented are one representative experiment. (B) Population doublings (PD) of NIH3T3 cells expressing an inducible form of a constitutively activated HIF-1α. Two independent clones were cultured in the presence or absence of doxycycline. Data are a representative experiment performed in triplicate ± SD. Inset shows a Western blot of HIF-1α expression in the transfected cells. Cells were treated with 200 μM desferrioxamine (cntrl), or exposed to vehicle control (−), or 0.5 μg/mL doxycycline (+). The doxycycline induction of transfected HIF-1α is comparable to the level of induction of endogenous HIF-1α in response to desferrioxamine. Total Akt was used as a loading control.