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. Author manuscript; available in PMC: 2008 Apr 13.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Feb 12;355(3):745–750. doi: 10.1016/j.bbrc.2007.02.025

Fig. 2. Overexpression of PACSIN3 in 3T3-L1 adipocytes promotes glucose uptake.

Fig. 2

(A) Schematic diagram of the PACSIN3 constructs expressed through adenoviral expression. (B) 3T3-L1 adipocytes at day 4–5 after differentiation were infected with equal viral titers of beta-galactosidase (βgal), mycWTPACSIN3 (WT), mycΔC354PACSIN3 (ΔC354), mycΔC329PACSIN3 (ΔC329) or mycΔN244PACSIN3 (ΔN244) constructs; 3 days post-infection, cells were incubated with or without 1 μM insulin for 30 min, followed by the measurement of 2-deoxy-glucose uptake. *, p < 0.05 and **, p < 0.01 compared to the corresponding βgal control by Student T-test. Values are the mean ± standard error from three independent experiments.