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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: Science. 2006 Apr 27;312(5776):1059–1063. doi: 10.1126/science.1127168

Fig. 4.

Fig. 4

Nuclear defects in cells from old individuals are caused by use of the LMNA exon 11 cryptic splice site. (A) Immunofluorescence microscopy on fibroblasts from an old healthy individual after treatment with either Exo11 oligonucleotide or a control scrambled oligonucleotide. Scale bar, 60 μm. (B and C) Quantitation of phenotypic rescue upon treatment with Exo11 oligonucleotide. (D) Quantitative RT-PCR analysis of HeLa cells and cell lines from young and old donors upon treatment with either Exo11 oligonucleotide or a control scrambled oligo-nucleotide. Values represent averages ± SD from a representative experiment. Statistical significances of the differences compared with mock-treated cells from the old donor are indicated. One asterisk indicates P < 0.1. Two asterisks indicate P < 0.05. (E) Quantitation of BrdU-positive cells in cell lines from young and old donors upon treatment with either Exo11 oligonucleotide or a control scrambled oligonucleotide. Values represent averages ± SD from three independent experiments.