Fig. 3.
Intramolecular effects of phosphorylation at Ser40 and phospho-mimetic mutations at Ser40 and 19 on phosphorylation of TH by Cdk5. (a) Histogram depicting quantitation of V/K-values for phosphorylation of the indicated forms of TH by Cdk5. Error bars (SEM) were derived from two separate determinations for each reaction. (b) Quantitative kinetic values for phosphorylation of the indicated forms of TH by Cdk5. *nd, not determined as described in the text.