TABLE 3.

Quantification of LRP mRNA levels in cultured granulosa cells pretreated with or without hLDL, hHDL₃, Bt₂cAMP, Bt₂cAMP plus hHDL₃,orBt₂cAMP plus hLDL

Additions	LRP mRNA Content
	arbitrary units/U 18S rRNA
None	0.81 ± 0.13
hHDL3 (500 μg/ml)	0.87 ± 0.16
hLDL (100 μg/ml)	0.80 ± 0.09
Bt2cAMP (2.5 mM)	0.89 ± 0.18
hHDL3 + Bt2cAMP	1.96 ± 0.42a
hLDL + Bt2cAMP	3.90 ± 0.48b

Results are means ± SEM of three separate experiments. Cultured granulosa cells were pretreated with or without Bt₂cAMP for 24 h. Subsequently, cells were incubated with the indicated concentrations of hHDL₃ or hLDL with or without Bt₂cAMP for an additional 24 h. Total cellular RNA preparations were used for the quantification of LRP mRNA and 18S rRNA levels using a sensitive RPA.

^aP < 0.04 versus Bt₂cAMP alone.

^bP < 0.002 versus Bt₂cAMP alone.