TABLE 5.
Chylomicron remnant-, hHDL3-, and Bt2cAMP-stimulated progestin production by cultured granulosa cells pretreated with Bt2cAMP plus hLDL
Addition | Progesterone (a) | 20α-Dihydroxyprogesterone (b) | Total Progestin (a + b) |
---|---|---|---|
Basal | 0.26 ± 0.03 | 0.56 ± 0.08 | 0.82 ± 0.06 |
Chylomicron remnants (80 μg/ml) | 0.49 ± 0.04 | 1.92 ± 0.34 | 2.41 ± 0.41 |
hHDL3 (500 μg/ml) | 1.39 ± 0.28 | 5.41 ± 0.811 | 6 .80 ± 1.02 |
Bt2cAMP (2.5 mM) | 30.9 ± 5.4 | 56.2 ± 5.1 | 96.0 ± 5.9 |
Chylomicron remnants + Bt2cAMP | 308 ± 52 | 331 ± 65 | 639 ± 94 |
hHDL3 + Bt2cAMP | 962 ± 52 | 909 ± 117 | 1871 ± 67 |
Results are means ± SEM of three separate experiments. Values are expressed as ng/μg DNA/24 h.