TABLE 6.

Effect of anti-SR-BI on hHDL₃-and rat VLDL-supported progestin production by cultured rat granulosa cells pretreated with Bt₂cAMP plus hLDL

Additions	Progestins
None (basal)	0.50 ± 0.12
Rat VLDL (80 μg/ml)	2.47 ± 0.47
Rat VLDL (80 μg/ml) + anti-SR-BI (50 μg IgG/ml)	2.18 ± 0.35
hHDL3 (500 μg/ml)	4.56 ± 0.54
hHDL3 (500 μg/ml) + anti-SR-BI (50 μg IgG/ml)	1.90 ± 0.49a
Bt2cAMP (2.5 mM)	48.7 ± 11.89
Bt2cAMP (2.5 mM) + rat VLDL (80 μg/ml)	549.3 ± 75.6
Bt2cAMP (2.5 mM) + rat VLDL (80 μg/ml) + anti-SR-BI (50 μg IgG/ml)	328.4 ± 81.0b
Bt2cAMP (2.5 mM) + hHDL3 (500 μg/ml)	1,205 ± 106
Bt2cAMP (2.5 mM) + hHDL3 (500 μg/ml) + anti-SR-BI (50 μg IgG/ml)	520.8 ± 91.9c

Results are means ± SEM of three separate experiments. Progestins are the sum of progesterone plus 20α-dihydroxyprogesterone.

^aP = 0.0211.

^bP = NS.

^cP = 0.0205.