Figure 6.
Mutation of Cysteines 744, 798 of the NR1 Subunit Abrogates Hypoxic Enhancement of NO-Mediated Inhibition NMDA-Evoked Currents
(A and B) Voltage-clamp traces from oocytes expressing NR1(C744A, C798A)/NR2A subunits under ambient air (A) and hypoxic (B) condition. SNOC produced dose-dependent inhibition of NMDA-evoked currents (200 μM NMDA plus 10 μM glycine) under ambient air conditions but hypoxia did not enhance this inhibition. (C) Inhibition of NMDA-evoked currents by NO, expressed as normalized responses to NMDA before SNOC application. Values are mean ± SEM (n = 4-5 oocytes).
