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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: J Biol Chem. 2005 Apr 18;280(24):23262–23272. doi: 10.1074/jbc.M503701200

FIG.3.

FIG.3

Reduction in ATM protein is required for TPA-mediated apoptosis. Apoptotic analysis of wild type ATM-transfected LN-CaP (WT8.4) compared with empty vector-transfected LNCaP (Control). TPA-treated cells were first preincubated for 16 h before irradiation at 20 Gy and then incubated for 48 or 72 h before apoptosis was quantified by bis-benzimide staining. The bars represent S.D. values. The inset shows ATM protein levels in WT8.4 and control cells after 16 h of TPA treatment, along with DNA-PKcs as a loading control. All studies were repeated three times.