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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Mol Cell Neurosci. 2007 Jan 17;34(3):453–467. doi: 10.1016/j.mcn.2006.11.022

Figure 1. Cdc42 activity is required for PTPμ to be a permissive growth substrate for nasal neurons.

Figure 1

Explants from E8 nasal retina were grown on alternating stripes of PTPμ or laminin only lanes (single stripe assay) and either Rac1, Cdc42 or RhoA dominant negative (DN) mutants were added using protein transduction via their TAT-tag. Control and RhoA-DN transduced nasal neurites cross onto a PTPμ substrate. Following addition of Rac1-DN or Cdc42-DN, however, nasal neurites are no longer able to cross onto PTPμ lanes. These results suggest that activation of Cdc42 is required for a permissive response to a PTPμ substrate. Quantitation of the degree of nasal avoidance of PTPμ lanes is shown following addition of dominant-negative mutants of the Rho GTPases to single stripe assays. The average degree of avoidance (mean +/− STDEV) was plotted for each condition as described in Experimental Methods.