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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: Nat Cell Biol. 2005 Jun 5;7(7):719–723. doi: 10.1038/ncb1274

Figure 1.

Figure 1

Argonaute proteins localize to mammalian P-bodies. (a) Myc-tagged Ago2 protein was expressed in U2-OS cells. Ago2 protein localized to discrete cytoplasmic foci by staining with FITC-conjugated anti-Myc. (b) Endogenous Ago2 protein was localized in U2-OS cells by staining with a rabbit anti-Ago2 antibody. (c) Argonaute proteins colocalized with either GFP- or Flag-tagged Dcp1a, a signature component of the mammalian P-bodies. Argonaute proteins were visualized using a Rhodamine-Red-conjugated anti-Myc. Dcp1a was visualized either by GFP or FITC-conjugated anti-Flag.