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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: Nat Cell Biol. 2005 Jun 5;7(7):719–723. doi: 10.1038/ncb1274

Figure 2.

Figure 2

Argonaute proteins bind components of mammalian P-bodies. Human 293T cells were transfected with Myc-tagged Ago1 or Ago2 in combination with either GFP-tagged Dcp1a or Flag-tagged Dcp2 expression plasmids (as indicated). (a) Ago1 or Ago2 (anti-Myc) or Dcp1a (anti-GFP) immunoprecipitates were western blotted with anti-Myc or anti-GFP antibodies as indicated. (b) Ago1 or Ago2 (anti-Myc) or Dcp2 (anti-Flag) immunoprecipitates were western blotted with anti-Myc or anti-Flag antibodies as indicated. (c) Extracts were treated with RNaseA before immunoprecipitation of Ago2 (anti-Myc) or Dcp1a (anti-GFP). Immunocomplexes were western blotted with anti-Myc or anti-GFP antibodies as indicated. (d) Extracts were treated with RNaseA before immunoprecipitation of Ago2 (anti-Myc) or Dcp2 (anti-Flag). Immunocomplexes were western blotted with anti-Myc or anti-Flag antibodies as indicated.