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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: Nat Cell Biol. 2005 Jun 5;7(7):719–723. doi: 10.1038/ncb1274

Figure 3.

Figure 3

Accumulation of Argonaute proteins in P-bodies requires an intact siRNA-binding domain. (a) Wild-type and mutant Ago2 proteins, Ago2-PAZ9 and Ago2-PAZ10 (as indicated), were expressed as Myc–epitope fusions in cells transfected with a luciferase siRNA. Western blotting with an anti-Myc antibody was used to measure protein expression (upper panel). Small RNA binding was measured by northern blotting of RNA extracted from immunocomplexes (middle panel). Nuclease activity was also measured against a complementary substrate (bottom panel). (b) Subcellular localization of wild-type and non-small RNA binding mutant Ago2 proteins was determined by immunostaining with anti-Myc antibodies. (c) Myc-tagged wild-type or mutant Ago2 proteins were co-expressed with GFP-tagged Dcp1a. Ago2 (anti-Myc) or Dcp1a (anti-GFP) immunoprecipitates were analysed by western blotting with anti-Myc or anti-GFP antibodies, as indicated.