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. Author manuscript; available in PMC: 2007 Apr 24.
Published in final edited form as: Nat Cell Biol. 2005 Jun 5;7(7):719–723. doi: 10.1038/ncb1274

Figure 4.

Figure 4

miRNA-dependent localization of target mRNAs to mammalian P-bodies. (a) Plasmids expressing the let-7 target, Myc–Ago2 protein and MS2–YFP–NLS were cotransfected into U2-OS cells. Reporter mRNA was visualized indirectly using the fusion protein. Ago2 protein was visualized using Rhodamine-Red-conjugated anti-Myc. (b) Analyses were identical to in a except that the target mRNA did not contain the lin-41 3′ UTR fragment. (c) The CXCR4 target was co-expressed with MS2–YFP–NLS and Myc–Ago2. Detection was as in a. (d) The CXCR4 reporter was co-expressed with MS2–YFP–NLS and with (upper) or without (lower) Myc–Ago2 in cells that were also transfected with the CXCR4 siRNA. Endogenous Ago2 was visualized with a rabbit anti-Ago2 antibody. Diagrammatic representation targets are shown next to each panel.